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KMID : 0380620200520010103
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Korean Journal of Food Science and Technology
2020 Volume.52 No. 1 p.103 ~ p.108
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Rapid detection of shiga-toxin producing E. coli by bacteriophage amplification assay
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Baek Da-Yun
Park Jong-Hyun
Cho Seok-Cheol
Lee Young-Duck
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Abstract
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Shiga toxin-producing Escherichia coli (STEC) is an important pathogenic bacteria and can cause severe foodborne disease. For STEC detection, conventional culture methods have disadvantages in the fact that conventional culture takes a long time to detect and PCR can also detect dead bacteria. To overcome these problems, we suggest a bacteriophage amplification assay, which utilizes the ability of bacteriophages to infect living cells and their high specificity. We used a combination of six bacteriophages infecting E. coli to make the bacteriophage cocktail and added ferrous ammonium sulfate as a virucidal agent to remove free-bacteriophages. When cherry tomato and paprika were artificially inoculated with the cocktail at a final concentration of around 3 log CFU/mL and were enriched for at least 5 h in mTSB broth with Novobiocin, approximately 2-3 log PFU/mL were detected through the bacteriophage amplification assay. Therefore, bacteriophage amplification assay might be convenient and a useful method to detect STEC in a short period of time.
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KEYWORD
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Bacteriophages, amplification, rapid detection, shiga-toxin, E. coli
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